27 Aug Reinventing Protein Sample Preparation
Technical Breakthrough for Protein Sample Preparation
Preparation of protein samples is traditionally done through solution-based methods such as RIPA buffer. This method has pitfalls that lead to protein loss, endogenous baseline shift and has low repeatability. Sonication shares many of these flaws, while more difficult and time consuming. The Minute™ Total Protein Extraction Kits are reinventing protein sample preparation. With a strong lysis buffer and a patented spin column-based filter, complete protein profiles can be easily achieved in just minutes! Take a look at the data comparison below:
Discover the Spin Column Advantages!
Fast Protein Extraction
– En Route to Replacing RIPA
– En Route to Replacing RIPA
- Super-Fast (as quick as 1 minute)
- All Living Organisms and Tissues/Cells
- Small Sample Size (~20µl)
- High Yield
- Complete Protein Profile
- Unaltered Endogenous Baseline
- Avoid Protein Loss Caused by RIPA
Cell Fractionation
– Using only a Tabletop Centrifuge
– Using only a Tabletop Centrifuge
- Plasma Membrane (Animal and Plant)
- Nuclear/Cytoplasmic
- Intact Nuclei
- Mitochondria
- Endoplasmic Reticulum
- Golgi (trans and cis)
- Endosome
- Lysosome
- Exosome
- Lipid Raft (Total and PM-derived)
- Proteasome (Cyto & Nucl)
- Plant Chloroplast
- Plant Microsomal Membrane
- Plant Cell Fractionation
