09 Jul Novel Tools for Viral Research

If you are involved in viral research, such as with the novel SARS-COV-2 virus, you may want to consider the Minute^TM Kits for cell fractionation and protein sample preparation to better understand COVID-19 and how it infects cells.

Cell Fractionation / Protein Trafficking
The spike protein of COVID-19 binds to the hACE2 receptor which is located on the plasma membrane. Viruses such as COVID-19 also take advantage of the cell’s trafficking pathways and use host cell machineries. They can also use organelles such as endosome, endoplasmic reticulum (ER) and Golgi apparatus to promote viral replication and evade antiviral responses. Our company has developed a line of products using patented spin column-based technologies that are the perfect tools to help with viral research involving membrane trafficking and organelle hijacking.

• Plasma Membrane Isolation and Cell Fractionation
• Endosome Isolation
• Endoplamic Reticulum (ER)
• Golgi Apparatus

Cytokine Release / Inflammation Response
Additionally, adipose tissue has shown to be a cytokine producing tissue. In the context of the COVID-19 virus, it is possible that adipose tissue may play a role in so called cytokine storm syndrome in obese patients. The Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (Cat# AT-022) may be useful to study the possible link between adipose tissue and cytokine release/ inflammation response.

Patented Spin Column-Based Technologies

Trusted technologies – widely literature cited

Fast Protein Extraction
– En Route to Replacing RIPA

• Super-Fast (as quick as 1 minute)
• Compatible with all living organisms and tissue types
• Small sample size (~20µl)
• Complete protein profile
• Unaltered endogenous baseline
• Consistent – no or greatly reduced variables
• Avoid protein loss caused by RIPA

Cell Fractionation
– Using only a Tabletop Centrifuge

• Plasma Membrane (Animal and Plant)
• Nuclear/Cytoplasmic
• Intact/Single Nuclei
• Mitochondria
• Endoplasmic Reticulum
• Golgi (Cis/Trans and Separated Trans Vesicles)
• Endosome
• Lysosome
• Exosome (Non-PEG)
• Lipid Raft (Total and PM-derived)
• Proteasome (Cyto & Nucl)
• Plant Chloroplast
• Plant Microsomal Membrane
• Plant Cell Fractionation