16 Aug [MCE] Organoids: Jump From 2D to 3D
Organoids are three-dimensional (3D) miniature structures cultured using human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs). Compared with traditional 2D cell culture, organoids can closelymimic the cellular heterogeneity, structure, and function of the original organ and are widely used in drug development, precision medicine, and regenerative medicine [1].
Different types of organoids require the addition of various components and ratios of growth factors for targeted induction, such as epidermal growth factor (EGF), Noggin, and R-spondin-1, which are then encapsulate into basement membrane matrix for organoid differentiation and expansion [2].
Figure 1. Schematic of the various organoids that can be grown
from PSCs and the employed developmental signals [2].
MedChemExpress Products for Organoids
| Category | Name | Description |
| Basement Membrane Matrix |
Basement Membrane Matrix (Phenol Red) | A natural basement membrane matrix mainly used for angiogenesis and tumor invasion assays. |
| Basement Membrane Matrix | A natural basement membrane matrix mainly used for angiogenesis and tumor invasion experiments while avoiding color interference in subsequent studies. | |
| Basement Membrane Matrix GFR (Phenol Red) | A natural basement membrane matrix contains lower levels of various cytokines than the standard type. This product is mainly used for organoid culture. | |
| Basement Membrane Matrix GFR | A natural basement membrane matrix which contains lessf various cytokines than the standard type. This product is mainly used for organoid culture while a voiding color interference in subsequent experiments. |
|
| Basement Membrane Matrix for Organoid Culture | A natural basement membrane matrix mainly used for organoid culture. | |
| Recombinant Proteins | Human EGF | A potent epidermal growth factor that stimulates the proliferation of epidermal cells and is used in wound healing. |
| Human Activin A | A multi-functional cytokine is a member of the TGF-β superfamily. | |
| Human Wnt3a | Promotes the Wnt/β-catenin signaling pathway by stabilization of intracellular beta-catenin levels. | |
| Human VEGF121 | A VEGF isoform splice variant cannot bind to heparin. | |
| Human Noggin | An antagonist of bone morphogenetic proteins (BMPs) binds with high affinity to hBMP-4. | |
| Inhibitors | Laduviglusib | A GSK-3α/β inhibitor, also a Wnt/β-catenin signaling pathway activator. |
| Y-27632 | A ROCK (Rho-kinase) inhibitor, primes human induced pluripotent stem cells (hiPSCs) selectively. |
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References:
[1] Signal Transduct Target Ther. 2022; 7: 168.
[2] Cell. 2016 Jun 16;165(7):1586-1597.
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