18 Jul [Inventory Biotechnologies] Minute™ Single Nucleus Isolation Kit for Tissues/Cells
Isolation and purification of nuclei from tissues and cultured cells are common lab practices. Isolated nuclei, especially single nucleus, are widely used for a variety of experiments such as FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and apoptosis research.
The traditional method for the isolation of nuclei is relatively simple. However, nucleus aggregation and formation of a high percentage of doublets are frequently encountered problems. This kit is specially designed to address these issues.
The protocol is simple, rapid, and very effective. A high percentage of the well-separated single nucleus can be obtained in about 30 min. In comparison to the traditional method, the kit requires less starting material and has a larger range of sample sizes (1-30 mg). The buffers contain a proprietary mix of detergents for efficient cell lysis.
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