[Invent Biotechnologies] Cell Isolation Kits

07 Jul [Invent Biotechnologies] Cell Isolation Kits

Cell isolation protocols do not need to be tedious. Invent Biotechnologies has developed the Minute™ Single Cell Isolation Kits to provide an easy, fast, spin column-based method. The combination of chemical and physical separation mechanisms leads to vastly improved results without requiring specialized instrumentation. Obtain a cell suspension with high integrity and viability in just minutes!

Minute™ Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps)

MinuteTM single cell isolation kit comprises optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to isolate single cells from fresh animal tissues rapidly, and it can also isolate nuclei from tissues briefly fixed with formaldehyde for chromosome immunoprecipitation (ChIP).

The tissue disaggregation buffers are formulated to disaggregate animal tissues gently. The buffers don’t contain any proteinases and EDTA that may adversely affect cell surface markers. Due to filter cartridges with pre-defined pore size and a specially formulated buffer system, single-cell suspension can be obtained from fresh tissues in less than 10 min. This kit is specifically designed for lymphoid tissues such as the spleen, thymus, and lymph node. For other tissues, the performance is tissue type-dependent.

Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps):

Obtaining quality cell suspension from fresh and/or frozen tissues is an important first step for subsequent analysis such as flow cytometry analysis (FACS) and nucleic acid purification.

Cell suspensions can be isolated from tissues by one or a combination of the following three mechanisms: chemical tissue dissociation, enzymatic digestion and physical separation. Many methods are tedious and time consuming. Most importantly, cells in the tissue, especially frozen tissue, are usually damaged and show low integrity and viability. It is very difficult to obtain high viability cell suspension from frozen tissues with high connective tissue contents such as liver, kidney and brain tissues.

Invent Biotechnologies has developed a simple, rapid, efficient, and instrument-free method for isolating cell suspension from fresh or frozen tissues using a combination of chemical tissue dissociation and physical separation mechanisms. The protocol can be finished in about 20 min with higher cell integrity and viability.