Ensure Reliable Internal Controls in WB

17 Oct Ensure Reliable Internal Controls in WB

Do you know the way the protein samples are obtained can significantly impact the detectability of housekeeping proteins in WB?

Housekeeping proteins such as actin, LC3I/II, GAPDH, tubulin and HSP90 are commonly used for Positive controls to establish the baseline for quantitative and qualitative Western blotting (WB). The detectability of these proteins is affected by many factors such as specificity of antibody and protein expression level as well as WB techniques. Following data shows protein extraction method can also affect the housekeeping protein detection:

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